Insert title here
    登录 | 注册 | 期刊登录 | 收藏
 
 高级组合检索
Analytical Chemistry 2014, Vol. 86 (2) :1269 -12697 doi:10.1021/ac403630a <<-上一篇 下一篇 ->>
MACRO: A Combined Microchip-PCR and Microarray System for;High-Throughput Monitoring of Genetically Modified Organisms
Ning, Shao;Jiang, Shi-Meng;Zhang, Miao;Wang, Jing;Guo, Shu-Juan;Li, Yang;Jiang, He-Wei;Liu, Cheng-Xi;Zhang, Da-Bing;Yang, Li-Tao;Tao, Sheng-Ce
[Ning, Shao; Guo, Shu-Juan; Li, Yang; Jiang, He-Wei; Liu, Cheng-Xi; Tao, Sheng-Ce] Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Key Lab Syst Biomed, Minist Educ, Shanghai 200240, Peoples R China.;[Jiang, Shi-Meng; Zhang, Miao; Wang, Jing; Zhang, Da-Bing; Yang, Li-Tao] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Collaborat Innovat Ctr Biosafety GMOs, Natl Ctr Mol Characterizat Genet Modified Organis, Shanghai 200240, Peoples R China.;[Ning, Shao; Guo, Shu-Juan; Li, Yang; Jiang, He-Wei; Liu, Cheng-Xi; Tao, Sheng-Ce] State Key Lab Oncogenes & Related Genes, Shanghai 200240, Peoples R China.;[Li, Yang; Jiang, He-Wei; Liu, Cheng-Xi; Tao, Sheng-Ce] Shanghai Jiao Tong Univ, Sch Biomed Engn, Shanghai 200240, Peoples R China.
Abstract: The monitoring of genetically modified organisms (GMOs) is a primary step of GMO regulation. However, there is presently a lack of effective and high-throughput methodologies for specifically and sensitively monitoring most of the commercialized GMOs. Herein, we developed a multiplex amplification on a chip with readout on an oligo microarray (MACRO) system specifically for convenient GMO monitoring. This system is composed of a microchip for multiplex amplification and an oligo microarray for the readout of multiple amplicons, containing a total of 91 targets (18 universal elements, 20 exogenous genes, 45 events, and 8 endogenous reference genes) that covers 97.1% of all GM events that have been commercialized up to 2012. We demonstrate that the specificity of MACRO is similar to 100%, with a limit of detection (LOD) that is suitable for real-world applications. Moreover, the results obtained of simulated complex samples and blind samples with MACRO were 100% consistent with expectations and the results of independently performed real-time PCRs, respectively. Thus, we believe MACRO is the first system that can be applied for effectively monitoring the majority of the commercialized GMOs in a single test.
Keywords: OLIGONUCLEOTIDE MICROARRAY MODIFIED MAIZE GM CROPS IDENTIFICATION
Insert title here
更多服务: 作者自存档 | 投稿导航 | 全球期刊检索 | 查找审稿人
关于我们 | 隐私申明 | 免责声明 | 意见反馈 | 合作服务
Magsci ©  版权所有
Copyright © 2010 - 2013 Magtech. All Rights Reserved.