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Chemical Society Reviews 2011, Vol. 40 (1) :221 -232 doi:10.1039/b907695f <<-上一篇 下一篇 ->>
Food analysis and food authentication by peptide nucleic acid;(PNA)-based technologies
Sforza, Stefano;Corradini, Roberto;Tedeschi, Tullia;Marchelli, Rosangela
[Sforza, Stefano; Corradini, Roberto; Tedeschi, Tullia; Marchelli, Rosangela] Univ Parma, Dept Organ & Ind Chem, I-43124 Parma, Italy.
Abstract: This tutorial review will address the issue of DNA determination in food by using Peptide Nucleic Acid (PNA) probes with different technological platforms, with a particular emphasis on the applications devoted to food authentication. After an introduction aimed at describing PNAs structure, binding properties and their use as genetic probes, the review will then focus specifically on the use of PNAs in the field of food analysis. In particular, the following issues will be considered: detection of genetically modified organisms (GMOs), of hidden allergens, of microbial pathogens and determination of ingredient authenticity. Finally, the future perspectives for the use of PNAs in food analysis will be briefly discussed according to the most recent developments.
Keywords: IN-SITU HYBRIDIZATION GENETICALLY-MODIFIED ORGANISMS 16S RIBOSOMAL-RNA EXCHANGE CHROMATOGRAPHIC ANALYSIS MISMATCH RECOGNITION DNA OLIGONUCLEOTIDES STRAND DISPLACEMENT ALLERGEN DETECTION CYSTIC-FIBROSIS POINT MUTATION
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