Magsci--全球跨学科期刊元数据存储平台
Insert title here
    登录 | 注册 | 期刊登录 | 收藏
 
 高级组合检索
Nature Biotechnology 2013, Vol. 31 (9) :822 -+ doi:10.1038/nbt.2623 <<-上一篇 下一篇 ->>
High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in;human cells
Foden, Jennifer A.1,2,3;Khayter, Cyd1,2,3;Fu, Yanfang1,2,3,4;Reyon, Deepak1,2,3,4;Sander, Jeffry D.1,2,3,4;Maeder, Morgan L.1,2,3,5;Joung, J. Keith1,2,3,4,5;
Foden, Jennifer A.1,2,3;Khayter, Cyd1,2,3;Fu, Yanfang1,2,3,4;Reyon, Deepak1,2,3,4;Sander, Jeffry D.1,2,3,4;Maeder, Morgan L.1,2,3,5;Joung, J. Keith1,2,3,4,5;
Abstract: Clustered, regularly interspaced, short palindromic repeat (CRISPR) RNA-guided nucleases (RGNs) have rapidly emerged as a facile and efficient platform for genome editing. Here, we use a human cell-based reporter assay to characterize off-target cleavage of CRISPR-associated (Cas) 9-based RGNs. We find that single and double mismatches are tolerated to varying degrees depending on their position along the guide RNA (gRNA)-DNA interface. We also readily detected off-target alterations induced by four out of six RGNs targeted to endogenous loci in human cells by examination of partially mismatched sites. The off-target sites we identified harbored up to five mismatches and many were mutagenized with frequencies comparable to (or higher than) those observed at the intended on-target site. Our work demonstrates that RGNs can be highly active even with imperfectly matched RNA-DNA interfaces in human cells, a finding that might confound their use in research and therapeutic applications.
Keywords: ZINC-FINGER NUCLEASES SYSTEMS ENDONUCLEASE GENERATION BACTERIA ARCHAEA MICE
Insert title here
更多服务: 作者自存档 | 投稿导航 | 全球期刊检索 | 查找审稿人
关于我们 | 隐私申明 | 免责声明 | 意见反馈 | 合作服务
Magsci ©  版权所有
Copyright © 2010 - 2013 Magtech. All Rights Reserved.