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Nature Methods 2013, Vol. 10 (10) :977 -+ doi:10.1038/NMETH.2598 <<-上一篇 下一篇 ->>
CRISPR RNA-guided activation of endogenous human genes
Linder, Samantha J.1,2,3;Cascio, Vincent M.1,2,3;Ho, Quan H.1,3;Maeder, Morgan L.1,2,3,4;Fu, Yanfang1,2,3,5;Joung, J. Keith1,2,3,4,5;
Linder, Samantha J.1,2,3;Cascio, Vincent M.1,2,3;Ho, Quan H.1,3;Maeder, Morgan L.1,2,3,4;Fu, Yanfang1,2,3,5;Joung, J. Keith1,2,3,4,5;
Abstract: Short guide RNAs (gRNAs) can direct catalytically inactive CRISPR-associated 9 nuclease (dCas9) to repress endogenous genes in bacteria and human cells. Here we show that single or multiple gRNAs can direct dCas9 fused to a VP64 transcriptional activation domain to increase expression of endogenous human genes. This proof-of-principle work shows that clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems can target heterologous effector domains to endogenous sites in human cells.
Keywords: SYSTEMS EXPRESSION
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