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BioMetals 2010, Vol. 23 (3 ) :543 -554 doi:10.1007/s10534-010-9298-0 <<-上一篇 下一篇 ->>
Probiotic Lactobacillus casei Expressing Human Lactoferrin Elevates Antibacterial Activity in the Gastrointestinal Tract
Hsiao-Ling Chen(1)   Yi-Wen Lai(2)   Chua-Shun Chen(2)   Te-Wei Chu(2)   Willie Lin(13)   Chih-Ching Yen(24)   Ming-Fong Lin(25)   Ming-Yu Tu(26)   Chuan-Mu Chen(24)   
1.Da-Yeh University Department of Molecular Biotechnology Changhwa 515 Taiwan
2.National Chung Hsing University Department of Life Sciences No. 250, Kuo Kuang Road Taichung 402 Taiwan
3.Fun Jen Catholic University Program of Technology Management Hsinchuang 242 Taiwan
4.China Medical University Hospital Taichung Taiwan
5.Taichung Hospital Department of Health Taichung 403 Taiwan
6.Taichung Armed Forces General Hospital Taichung 411 Taiwan
Abstract: In this study, Lactobacillus casei was used to deliver and express human lactoferrin (hLF) to protect the host against bacterial infection. Full-length hLF cDNA was cloned into a Lactobacillus-specific plasmid to produce the L. casei transformants (rhLF/L. casei). Antimicrobial activity of recombinant hLF was examined in inhibition of bacteria growth in vitro. A mouse model was established to test in vivo antibacterial activity and protective effect of orally-administered probiotic L. casei transformant in the gastrointestinal tract. Trials were conducted in which animals were challenged with E. coli ATCC25922. E. coli colony numbers in duodenal fluid from the group fed with rhLF/L. casei were significantly lower than those of the group fed with wild-type L. casei or placebo (P < 0.01). Histopathological analyses of the small intestine, showed both decreased intestinal injury and increased villi length were observed in the mice fed with rhLF/L. casei as compared with the control groups (P < 0.01). Our results demonstrate that L. casei expressing hLF exhibited antibacterial activity both in in vitro and in vivo. It also provides a potentially large-scale production of hLF as applications for treatment of infections caused by clinically relevant pathogens.
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